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Some web applications suddenly asking for network login.

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I've got about a dozen intranet web apps that are set to deny anonymous login.  Back in December they all worked fine.  Starting today (1/5/2015) some of them still work the same but a few of them are now demanding a network login and rejecting any valid user ID/Password given.  If I publish one of the broken web apps to a test directory set up exactly the same as the broken web app on the same server, it will run on the test directory and app pool just fine.  We have yet to find any differences at all between the two.  Republishing, recycling the app pool, replacing all references in the project, all fail to resolve the problem.  Opening the failing apps from the web server local in a browser works fine.  It only seems to fail if it is browsed to from a client machine.  Client machines are a mix of physical desktops and VDIs on VMware.  The server is Virtual on VMware and running Win Server 2008 R2 IIS 7.5. 

Web.config looks like this:

<?xmlversion="1.0"?>
<configuration>
  <system.web>
    <customErrorsmode="Off"/>
    <compilationdebug="true"strict="false"explicit="true"targetFramework="4.5">
      <assemblies>
        <addassembly="eWorld.UI, Version=2.0.6.2393, Culture=neutral, PublicKeyToken=24D65337282035F2"/>
      </assemblies>
    </compilation>
    <authenticationmode="Windows"/>
    <sessionStatemode="InProc"timeout="600"/>
    <authorization>
      <denyusers="?"/>
    </authorization>
    <pagescontrolRenderingCompatibilityVersion="4.0"/>
  </system.web>
</configuration>

Anyone have any suggestions?


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